Terminology Service for NFDI4Health
All terms in BAO
| Label | Id | Description |
|---|---|---|
| medulloblastoma | DOID_0050902 | [An infratentorial cancer that is located_in the lower part of the brain and is a type of primitive neuroectodermal tumor.] |
| Renilla-Glo luciferase assay system (Promega) | BAO_0140042 | [A bioluminescent assay sytem for detecting sea pansy (Renilla reniformis) luciferase, an alternative to the firefly (Photinus pyralis) systems.] |
| nephrosis | DOID_2527 | |
| Ub-CHOP2-Reporter-Kit (LifeSensors Inc.) | BAO_0140043 | [An assay for deubiquitination activity, employing a reporter enzyme fused to ubiquitin (making the reporter catalytically inactive). Following cleavage of the Ub-reporter system by the isopeptidase, the free reporter can act upon its fluorescent substrate. ] |
| splenic disease | DOID_2529 | |
| PathHunter U2OS Keap1-NRF2 Nuclear Translocation Assay (DiscoverX) | BAO_0140040 | [An assay system employing enzyme fragment complementation (two fragments of the beta-galactosidase enzyme), applied in U2OS cells engineered to express two appropriately tagged proteins. Inhibition of Keap1-NRF2 interaction results in Nrf2 nuclear translocation, and susbequent complementation and formation of a functional enzyme, detected using a chemiluminescent substrate of beta-galactosidase.] |
| CellTiter-Fluor Cell Viability Assay Kit | BAO_0140046 | [The CellTiter-Fluor Cell Viability Assay measures the relative number of viable cells in a population. The assay is based on measurement of a conserved and constitutive protease activity within live cells and therefore serves as a biomarker of cell viability. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. (description adapted from vendor Promega)] |
| Sufu-KO-LIGHT cell | CLO_0037222 | [Sufu-KO-LIGHT cells were derived from Sufu knockout mouse embryonic fibroblasts according to the method of Chen et al. [Genes Dev. 2009;23(16): 1910-28.] The cells were co-transfected with the zeocin resistance vector (pVgRXR, Invitrogen) and a firefly luciferase reporter driven by eight Gli binding sites and a γ-crystallin basal promoter (8XGHBS-FL). Sufu-KO-LIGHT lacks Sufu and exhibits constitutive Hh target gene expression in response to endogenous Gli activators.] |
| EC 6.6.* (N-metal bond-forming ligase) inhibitor | CHEBI_76708 | |
| EC 6.* (ligase) inhibitor | CHEBI_75603 | |
| RD-HGA16 | CLO_0037221 | |
| Ub-G76V-GFP/HeLa | CLO_0037223 | [A Hela-derived cell line cell that expresses ubiquitinated GFP (Green Fluroescent Protein)] |
| alternate organism assay | BAO_0001066 | [This assay is performed with a different organism than that was tested in primary or confirmatory assays.] |
| selectivity assay | BAO_0000478 | [Assay employed to elucidate the specificity of biologically active chemical entities towards one or more targets that are (closely) related to the (intended) primary target.] |
| alternate cell line assay | BAO_0001065 | [This assay is performed with a different cell line than that was tested in primary or confirmatory assays.] |
| alternate target assay | BAO_0001064 | [This assay is performed with a different but a related target (usually from the same protein family) than that was tested in primary or confirmatory assays, e.g., SIP1 instead of SIP3.] |
| counter screening assay | BAO_0000803 | [An assay run to eliminate those hits from the primary and confirmatory assay stages that are not of interest, i.e. do not act via the MMOA of interest; for example compounds that are assay artifacts (e.g. fluorescent), promiscuously active, toxic. ] |
| HeLa-R5-16 | CLO_0037220 | [HeLa-R5-16 (gift from Roche Palo Alto, LLC) is a derivative of HeLa cells, engineered to express the Envelope protein (Env) from the CCR5-tropic HIV-1 clinical isolate 92US715, and also to express the Tat protein from HIV-1 NL43] |
| empirical scoring function | BAO_0002394 | |
| AutoDock3 scoring function | BAO_0002395 |