Terminology Service < Semantic Lookup Platform

All terms in BAO

Label	Id	Description
disease by infectious agent	DOID_0050117	[A disease that is the consequence of the presence of pathogenic microbial agents, including pathogenic viruses, pathogenic bacteria, fungi, protozoa, multicellular parasites, and aberrant proteins known as prions.]
ischemic bone disease	DOID_0080008	[A bone disease that results_in an interruption of blood supply located_in bone.]
EC 1.1.1.184 [carbonyl reductase (NADPH)] inhibitor	CHEBI_73136
EC 2.7.11.12 (cGMP-dependent protein kinase) inhibitor	CHEBI_85113
fluorescence polarization	BAO_0000003	[Fluorescence polarization (FP) measurements are based on the assessment of the rotational motions of species. It is used to measure binding interactions. When linear polarized light is used to excite fluorophores, only those aligned with the plane of polarization will be excited. For fluorophores attached to small, rapidly rotating molecules, the initially photoselected orientational distribution becomes randomized prior to emission, resulting in low fluorescence polarization. But binding of the low molecular weight tracer to a large, slowly rotating molecule results in high fluorescence polarization.]
fluorescence method	BAO_0000046	[Detection techniques that use the principles of fluorescence, whereby incident light excites a fluorophore which then emits light at lower energy (higher wavelength). The emitted light is typically from the visible portion of the UV-Visible spectrum.]
homogeneous time resolved fluorescence	BAO_0000002	[Homogenous time resolved fluorescence (HTRF) is a technology which combines standard FRET technology with the time-resolved measurement of fluorescence. Through this, HTRF eliminates the short-lived background fluorescence. Introducing a time delay (50-150 micro seconds) between the system excitation and fluorescence measurement, which allows the signal to be cleared of all non-specific short lived emissions.]
fluorescence resonance energy transfer	BAO_0000001	[Fluorescence resonance energy transfer (FRET) is based on the transfer of energy between two fluorophores, a donor and an acceptor, when in close proximity. The energy transfer is directed from higher-energy donor fluorophore to lower-energy acceptor fluorophore of labeled protein pairs. Target protein pairs are likely to exhibit FRET if they are no more than 10 nm apart.]
90 percent inhibition	BAO_0002667	[90% reduction of a predefined stimulus. Unit of Measure is always % when normalized to the dynamic range of the assay.]
blepharitis	DOID_9423	[An eyelid disease that is characterized by often chronic inflammation of the eyelid, generally the part where eyelashes grow.]
eyelid disease	DOID_530	[An adnexa disease that is located_in the eyelid.]
intracranial hypertension	DOID_9428
signal transducer and activator of transcription 5 protein inhibitor	CHEBI_87786
ADMET	BAO_0000009	[ADMET stands for absorption, distribution, metabolism, excretion and toxicity. Admet assays are performed for verifying the bio-availability, toxicity, metabolic stability, and drug-drug interaction potential of drugs: They quantify absorption of drugs in the intestine which is dependent on their solubility; distribution which ascertains their binding to plasma proteins, central nervous system penetration; metabolism where the in vivo clearance is monitored; and finally, toxicity in terms of inhibition of liver cytochrome p450 enzymes or hERG which could cause drug-drug interactions and cardiac arrhythmias, respectively.]
bioassay specification	BAO_0000026	[Description of an experiment carried out for the purpose of screening a perturbing agent in a biological system, measuring the effect of the agents using specified technology, and arriving at endpoints that satisfy particular criteria.]
luciferase reporter gene assay	BAO_0002661
reporter gene assay	BAO_0003006	[Reporter gene assay measures the gene expression from a reporter gene. The reporter gene is inserted under the control of a foreign promoter or an artificial regulatory element of interest. Reporters include luciferase, beta galactosidase, beta lactamase, chloramphenicol acetyl transferase, or a fluorescent protein.]
nucleosome format	BAO_0000007	[They are the basic repeating units of the eukaryotic chromatin consisting of DNA wound around a histone protein core.]
subcellular format	BAO_0000220	[subcellular organelles / component (not individual proteins) obtained via cell lysis and differential centrifugation (or potentially other method of purification / extraction of the organelles)]
percent cytotoxicity	BAO_0000006	[It is a measure of the number of dead cells in a culture well. We consider it as a subclass of 'percent inhibition', because cell death is induced by inhibition of vital cellular processes and percent cytotoxicity increases as the number of dead cells increases. It can be estimated in the compound treated wells in comparison to vehicle-treated control wells and is expressed as a percent. The compound treatment is preferably performed in a short window of time to avoid masking the effect due to cell proliferation. Compound cytotoxicity is an important parameter to measure when developing potential human therapeutics. Cytotoxicity is determined as a measure of radioisotope (3H thymidine or 51Cr) release, lactate dehydrogenase release from damaged cells, tetrazolium salt and alamar blue reduction, fluorescent dyes that selectively stain live or dead cells, and decrease in ATP content. ATP levels are detected using a luminescence based assay kit such as CellTiter-Glo (Promega). ATP values higher than controls indicate proliferation and cultures with ATP concentrations lower than controls indicate cytotoxicity. Percent cytotoxicity = 100-percent viability]