Terminology Service < Semantic Lookup Platform

All terms in ONE

Label	Id	Description
Southwestern blot assay	OBI_0002173	[A direct binding assay that detects interactions of labeled DNA with immobilized protein on a membrane.]
immunocytochemistry	OBI_0002174	[An immuno staining assay in which samples of intact cells are examined that have had most, if not all, of their surrounding extracellular matrix removed]
ATP bioluminescence assay	OBI_0002175	[An analyte assay that detects ATP concentration through light intensity when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions, and molecular oxygen.]
T cell proliferation	GO_0042098	[The expansion of a T cell population by cell division. Follows T cell activation.]
electrophysiology assay	OBI_0002176	[An assay that measures the electrical properties of biological cells or tissues. Typically, this assay will generate measurements of voltage changes or electric current (or other associated variables such as impedence or capacitance).]
intra cellular electrophysiology recording assay	OBI_0000447	[An electrophysiology recording assay where the recording location of the electrode is intracellular]
whole-cell patch clamp assay	OBI_0002178	[A patch-clamp assay where the electrode is left in place on the cell, as in cell-attached recordings, but the membrane patch has been perforated, providing access from the interior of the pipette to the intracellular space of the cell. Measurements made with this technique involve recording currents through multiple channels simultaneously, over the membrane of the entire cell.]
inside-out patch clamp assay	OBI_0002180	[A patch-clamp assay where a patch of the membrane is attached to the patch pipette, detached from the rest of the cell, and the cytosolic surface of the membrane is exposed to the external media, or bath. This provides the experimenter has access to the intracellular surface of the membrane via the bath and can manipulate the environment at the intracellular surface of single ion channels. For example, channels that are activated by intracellular ligands can then be studied through a range of ligand concentrations.]
outside-out patch clamp assay	OBI_0002181	[A patch-clamp assay where a patch of the membrane is attached to the patch pipette. In this configuration, the external surface of the cell membrane is exposed as the outside of the membrane patch relative to the patch electrode. An outside-out patch starts with a gigaohm seal in a whole-cell recording configuration. The electrode is slowly withdrawn from the cell, until a fragment of membrane bulges away from the cell, which detaches and reforms as a convex membrane on the end of the electrode, with the original external surface of the membrane facing outward from the electrode. This provides the experimenter with access to the extracellular surface of the membrane via the bath and can manipulate the environment at the extracellular surface of single ion channels.]
performing a clinical assessment	OBI_0600003	[A protocol application during which a series of tests are made of a patient leading to determination of disease state, or condition.]
performing a diagnosis	OBI_0000988	[The interpretation of the information available about bodily features (clinical picture) of a patient resulting in a diagnosis]
diagnosis textual entity	OBI_0000075	[diagnosis is an assessment of a disease or injury, its likely prognosis and treatment.]
human subject enrollment	OBI_0600004	[A planned process with the objective to obtain a population of human subjects to participate in an investigation by determining eligibility of subjects and obtaining informed consent.]
selection	OBI_0001928	[A planned process which results in the creation of group of entity from a larger group by the application of predefined criteria.]
informed consent process	OBI_0000810	[A planned process in which a person or their legal representative is informed about key facts about potential risks and benefits of a process and makes a documented decision as to whether the person in question will participate.]
killing	OBI_0600006	[A protocol application in which an organism is intentionally put to death]
adding substance to cell culture	OBI_0600000	[is process in which a material substance is added to a cell culture]
tumor grading	OBI_0600002	[An assay that determines the grade (severity/stage) of a tumor sample, used in cancer biology to describe abnormalities/qualities of tumor cells or tissues. Values can be described by terms from NCI Thesaurus.]
voltage clamp assay	OBI_0002182	[An cellular electrophysiology assay where the membrane potential of a cell is controlled by the experimentalist. This is accomplished through a feedback mechanism where any change in membrane potential is countered by permitting electrical current to flow into or out of the cell.]
two electrode voltage clamp assay	OBI_0002183	[The two electrode voltage clamp (TEVC) method utilizes two low-resistance pipettes, one sensing voltage and the other injecting current. The microelectrodes are filled with conductive solution and inserted into the cell to artificially control membrane potential. The membrane acts as a dielectric as well as a resistor, while the fluids on either side of the membrane function as capacitors.[9] The microelectrodes compare the membrane potential against a command voltage, giving an accurate reproduction of the currents flowing across the membrane. Current readings can be used to analyze the electrical response of the cell to different applications. This technique is mainly used in the Oocyte preparation.]