Terminology Service < Semantic Lookup Platform

All terms in ONE

Label	Id	Description
IHC slide staining	OBI_0002123	[A staining using labeled antibodies, i.e. the analytical part of immunohistochemistry. The staining is applied to fixed specimens on slides to identify biomarkers in tissues.]
respiratory system fluid/secretion	UBERON_0006538	[A portion of organism substance that secreted_by a respiratory system.]
methylation-specific polymerase chain reaction assay	OBI_0001684	[An assay that uses initial modification of DNA by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA.]
amplification of intermethylated sites assay	OBI_0001685	[An assay that estimates genome-wide DNA methylation and measures methylation of DNA sequences. AIMS is based on the differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers followed by restrained PCR amplification of methylated sequences.]
DNA methylation profiling assay	OBI_0000634	[An assay that measures the state of methylation of DNA molecules using genomic DNA collected from a material entity using a range of techniques and instrument such as DNA sequencers and often relying on treatment with bisulfites to ensure cytosine conversion.]
high molecular weight DNA extract	OBI_0000834	[The output of an extraction process in which DNA molecules above a molecular weight cutoff are purified in order to exclude DNA from organellas.]
nucleic acid hybridization	OBI_0302903	[a planned process by which totally or partially complementary, single-stranded nucleic acids are combined into a single molecule called heteroduplex or homoduplex to an extent depending on the amount of complementarity.]
labeled nucleic acid extract	OBI_0001143	[a labeled specimen that is the output of a labeling process and has grain labeled nucleic acid for detection of the nucleic acid in future experiments.]
contact representative role	OBI_0001687	[A role inhering in a person who represents an institution, organization, or service provider and realized when communication is directed at them about the entity they represent.]
Homo sapiens	NCBITaxon_9606
cytochalasin-induced inhibition of actin polymerization assay	OBI_0001689	[An assay which uses compound cytochalasin (CHEBI: 23528) to block actin polymerization-dependent cell motility (GO:0070358) and actin filament polymerization (GO:0030041).]
cytochalasin	CHEBI_23528
hypothesis generating investigation	OBI_0000356	[is an investigation in which data is generated and analyzed with the purpose of generating new hypothesis]
Sos-recruitment assay	OBI_0001680	[A binding assay that detects interacting proteins for a targeted domain where mammalian guanyl nucleotide exchange factor (GEF) Sos is recruited to the Saccharomyces cerevisiae plasma membrane harboring a temperature-sensitive Ras GEF, Cdc25-2, allowing growth at the nonpermissive temperature using the Sos recruitment system, a kind of yeast 2 hybrid system.]
hypothesis driven investigation	OBI_0000355	[is an investigation with the goal to test one or more hypothesis]
yeast one-hybrid assay	OBI_0001681	[An assay that determines protein DNA interactions using a single fusion protein in which the activating domain is linked directly to the binding domain.]
bacterial one-hybrid assay	OBI_0001682	[An assay that identifies the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences, is cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will recruit RNA polymerase to the promoter and activate transcription of the reporter genes in that clone. The two reporter genes, HIS3 and URA3, allow for positive and negative selections, respectively. At the end of the process, positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence]
chromosome organization assay by fluorescence in-situ hybridization	OBI_0001683	[An in-situ hybridization assay that uses fluorescence to detect chromosomal integrity]
T cell receptor co-receptor CD8	PR_000025402	[A protein complex that is a membrane-bound heterodimeric co-receptor for MHC class-I antigen/T-cell receptor interaction.]
biological activity assay measuring epitope specific immune complex formation	OBI_0001695	[A B cell epitope specific activation of additional immune response in vitro assay that detects agglutination.]